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- Table of Contents
RAB27B antibody (A04890-2) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of RAB27B using anti-RAB27B antibody (A04890-2).
RAB27B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
RAB27B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-RAB27B Antibody Picoband®
RAB27B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
RAB27B antibody was positively detected by WB in:
human HEL whole cell, human RT4 whole cell, human Hacat whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RAB27B antibody was positively detected by IHC in:
human prostatic cancer tissue, mouse stomach tissue, rat stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
RAB27B antibody was positively detected by IF in:
human bladder cancer tissue
RAB27B antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-RAB27B Antibody Picoband®
View all RAB27B Antibodies
View all RAB27B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human RAB27B recombinant protein (Position: G4-C218).
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
24.6 kDa
Background of RAB27B
Enables guanyl ribonucleotide binding activity; myosin V binding activity; and protein domain specific binding activity. Involved in multivesicular body sorting pathway and positive regulation of exocytosis. Located in Golgi stack and cytoplasmic vesicle.
Cite This Product
Anti-RAB27B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04890-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
97%
High Homology
Rabbit
96%
High Homology
Mouse
94%
High Homology
Sheep
94%
High Homology
Rat
93%
High Homology
Cow
93%
High Homology
Zebrafish
83%
Moderate Homology
Drosophila
54%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RAB27B antibody
Immunohistochemistry (IHC) protocol for RAB27B antibody
Immunofluorescence (IF) protocol for RAB27B antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RAB27B antibody
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Product Promise
We promise Anti-RAB27B Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A04890-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026