Anti-RAB3A Antibody

Product Name

Anti-RAB3A Antibody

View all RAB3A Antibodies

SKU/Catalog Number

M03463

Size

50 µl

Description

Boster Bio Anti-RAB3A Antibody (Catalog # M03463). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RAB3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03463)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1531CT562.14.57

Isotype

IgG1,k

Immunogen

This RAB3A antibody is generated from a mouse immunized with a recombinant protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03463 is reactive to RAB3A in Human, Mouse, Rat

Applications

M03463 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24.984kDa

Background of RAB3A

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000
FC: 1:25

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal phaeochromocytoma cell line) cells labeling RAB3A with M03463 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on PC-12 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

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Anti-RAB3A Antibody at 1:500 dilution + human brain lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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All lanes : Anti-RAB3A Antibody at 1:2000 dilution
Lane 1: mouse brain lysate
Lane 2: rat brain lysate
Lysates/proteins at 20 g per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Overlay histogram showing PC-12 cells stained with M03463 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03463, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For RAB3A (Source: Uniprot.org, NCBI)

Alternative Names

RAB3A, member RAS oncogene family; RAS-associated protein RAB3A; ras-related protein Rab-3A RAB3A RAB3A, member RAS oncogene family ras-related protein Rab-3A|RAS-associated protein RAB3A

For more info on RAB3A, check out the RAB3A Infographic

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In this infographic, you will see the following information for RAB3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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