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Product Info Summary
| SKU: | M09431 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-RAB3B Antibody
SKU/Catalog Number
M09431
Size
50 µl
Description
Boster Bio Anti-RAB3B Antibody (Catalog # M09431). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAB3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09431)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1543CT354.60.92
Isotype
IgG1,k
Immunogen
This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B.
Reactive Species
M09431 is reactive to RAB3B in Human, Mouse, Rat
Calculated molecular weight
24.8 kDa
Background of RAB3B
Protein transport. Probably involved in vesicular traffic (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09431 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RAB3B Antibody at 1:2000 dilution
Lane 1: mouse brain lysate
Lane 2: rat brain lysate
Lane 3: SW480 whole cell lysate
Lane 4: human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M09431 staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing HepG2 cells stained with M09431 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M09431, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-RAB3B Antibody (M09431)
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