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Product Info Summary
| SKU: | M04643-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Rab4 RAB4A Rabbit Monoclonal Antibody
SKU/Catalog Number
M04643-1
BM4197 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rab4 RAB4A Rabbit Monoclonal Antibody catalog # M04643-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab4 RAB4A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04643-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCI-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rab4
Reactive Species
M04643-1 is reactive to RAB4A in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04643-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Rab4 expression in (1)MCF7 cell lysates (2)HeLa cell lysate.
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Immunofluorescent analysis of Hela cells, using Rab4 Antibody .
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Specific Publications For Anti-Rab4 RAB4A Rabbit Monoclonal Antibody (M04643-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Rab4 RAB4A Rabbit Monoclonal Antibody
Question
We need to test anti-Rab4 Rabbit Monoclonal antibody M04643-1 on mouse vagina for research purposes, then I may be interested in using anti-Rab4 Rabbit Monoclonal antibody M04643-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The products we sell, including anti-Rab4 Rabbit Monoclonal antibody M04643-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-Rab4 Rabbit Monoclonal antibody M04643-1 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
The anti-Rab4 Rabbit Monoclonal antibody (M04643-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-11
Question
See attached the WB image, lot number and protocol we used for vagina using anti-Rab4 Rabbit Monoclonal antibody M04643-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-13
Question
Would M04643-1 anti-Rab4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Jha
Verified customer
Asked: 2013-09-20
Answer
You can see on the product datasheet, M04643-1 anti-Rab4 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-20
