Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9)

Boster Bio Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9) catalog # M01891-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01891-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9)

See all RAB5A products

SKU/Catalog Number

M01891-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9) catalog # M01891-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01891-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

3.00E+09

Isotype

IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01891-1 is reactive to RAB5A in Human, Mouse, Rat

Applications

M01891-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For RAB5A (Source: Uniprot.Org, NCBI)

Uniprot ID

P20339

Gene ID

5868

Gene Name

RAB5A

Full Name

Ras-related protein Rab-5A

Weight

23.659kDa

Superfamily

small GTPase superfamily

Alternative Names

RAB5A, member RAS oncogene family; RAB5RAS-associated protein RAB5A; ras-related protein Rab-5A RAB5A RAB5 RAB5A, member RAS oncogene family ras-related protein Rab-5A|RAS-associated protein RAB5A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RAB5A, check out the RAB5A Infographic

RAB5A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RAB5A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-Rab5/RAB5A Antibody Picoband™ (monoclonal, 3E9)

Question

Can you help my question with product M01891-1, anti-Rab5/RAB5A antibody (monoclonal, 3E9). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-16

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-19

Question

Would M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

As indicated on the product datasheet, M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-04

Question

We have seen staining in human substantia nigra. What should we do? Is anti-Rab5/RAB5A antibody (monoclonal, 3E9) supposed to stain substantia nigra positively?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

From literature substantia nigra does express RAB5A. From Uniprot.org, RAB5A is expressed in substantia nigra, brain, brain subthalamic nucleus, cervix placenta, cervix carcinoma, melanoma, among other tissues. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2019-09-30

Question

We are currently using anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

The anti-Rab5/RAB5A antibody (monoclonal, 3E9) (M01891-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-08

Question

I see that the anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-05

Question

Would anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 work for Flow Cytometry with substantia nigra?

Verified Customer

Verified customer

Asked: 2019-04-16

Answer

According to the expression profile of substantia nigra, RAB5A is highly expressed in substantia nigra. So, it is likely that anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 will work for Flow Cytometry with substantia nigra.

Boster Scientific Support

Answered: 2019-04-16

Question

My colleagues were satisfied with the WB result of your anti-Rab5/RAB5A antibody (monoclonal, 3E9). However we have observed positive staining in substantia nigra cell membrane using this antibody. Is that expected? Could you tell me where is RAB5A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-11-08

Answer

From what I have seen in literature, substantia nigra does express RAB5A. Generally RAB5A expresses in cell membrane. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2018-11-08

Question

I was wanting to use your anti-Rab5/RAB5A antibody (monoclonal, 3E9) for Flow Cytometry for human substantia nigra on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human substantia nigra identification?

K. Zhao

Verified customer

Asked: 2018-01-17

Answer

As indicated on the product datasheet, M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9) has been validated for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human substantia nigra in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-17

Question

Is a blocking peptide available for product anti-Rab5/RAB5A antibody (monoclonal, 3E9) (M01891-1)?

M. Johnson

Verified customer

Asked: 2017-11-23

Answer

We do provide the blocking peptide for product anti-Rab5/RAB5A antibody (monoclonal, 3E9) (M01891-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-23

Question

I am interested in to test anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 on human substantia nigra for research purposes, then I may be interested in using anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Brown

Verified customer

Asked: 2017-02-10

Answer

The products we sell, including anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-02-10

Question

Is there a BSA free version of anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 available?

D. Lewis

Verified customer

Asked: 2016-07-12

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-07-12

Question

Is this M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9) reactive to the isotypes of RAB5A?

R. Evans

Verified customer

Asked: 2015-06-15

Answer

The immunogen of M01891-1 anti-Rab5/RAB5A antibody (monoclonal, 3E9) is A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-06-15

Question

Our lab used your anti-Rab5/RAB5A antibody (monoclonal, 3E9) for Flow Cytometry on cervix carcinoma last year. I am using human, and I plan to use the antibody for WB next. My lab would like examining cervix carcinoma as well as substantia nigra in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

K. Moore

Verified customer

Asked: 2015-03-04

Answer

I looked at the website and datasheets of our anti-Rab5/RAB5A antibody (monoclonal, 3E9) and it appears that M01891-1 has been tested on human in both Flow Cytometry and WB. Thus M01891-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-03-04

Question

Please see the WB image, lot number and protocol we used for substantia nigra using anti-Rab5/RAB5A antibody (monoclonal, 3E9) M01891-1. Please let me know if you require anything else.

E. Wu

Verified customer

Asked: 2014-03-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-20

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M01891-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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