Product Info Summary
| SKU: | PA2281 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rab9/RAB9A Antibody Picoband®
SKU/Catalog Number
PA2281
BA3539-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab9/RAB9A Antibody catalog # PA2281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab9/RAB9A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2281)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2281 is reactive to RAB9A in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
22.8 kDa
Background of RAB9A
Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What's more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2281 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, HELA Whole Cell, PC12 Whole Cell, NIH Whole Cell, A431 Whole Cell, 293T Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Rab9 antibody, PA2281, All Western blotting
All lanes: Anti-RAB9A(PA2281) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: NIH Whole Cell Lysate at 40ug
Lane 6: A431 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Specific Publications For Anti-Rab9/RAB9A Antibody Picoband® (PA2281)
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