Anti-RAC1 Antibody

Product Name

Anti-RAC1 Antibody

View all Rac1 Antibodies

SKU/Catalog Number

M00041-1

Size

80 µl

Description

Boster Bio Anti-RAC1 Antibody (Catalog # M00041-1). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RAC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00041-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1301CT276.121.104

Isotype

IgG2b

Immunogen

This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00041-1 is reactive to RAC1 in Human, Rat

Applications

M00041-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.45kDa

Background of Rac1

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:25
FC: 1:100

Validation Images & Assay Conditions

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Western blot analysis of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right), using RAC1 Antibody. M00041-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

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Immunohistochemical analysis of paraffin-embedded H. skin section using RAC1 (Cat#M00041-1). M00041-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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Immunohistochemical analysis of paraffin-embedded H. stomach section using RAC1 (Cat#M00041-1). M00041-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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Flow cytometric analysis of U-87 MG cells using RAC1 (green, Cat#M00041-1) compared to an isotype control of mouse IgG2b (blue). M00041-1 was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Gene/Protein Information For RAC1 (Source: Uniprot.org, NCBI)

Alternative Names

Cell migration-inducing gene 5 protein; MGC111543; p21-Rac1; p21-Rac1migration-inducing gene 5; Rac1; Ras-like protein TC25; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1); ras-related C3 botulinum toxin substrate 1; rho family, small GTP binding protein Rac1; TC25; TC-25 RAC1 MIG5, MRD48, Rac-1, TC-25, p21-Rac1 Rac family small GTPase 1 ras-related C3 botulinum toxin substrate 1|cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

For more info on RAC1, check out the RAC1 Infographic

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In this infographic, you will see the following information for RAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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