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Product Info Summary
| SKU: | M01714 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-RAC2 Antibody (C-term)
SKU/Catalog Number
M01714
Size
80 µl
Description
Boster Bio Anti-RAC2 Antibody (C-term) (Catalog # M01714). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAC2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01714)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RAC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 178-192 amino acids from the C-terminal region of human RAC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01714 is reactive to RAC2 in Human, Mouse
Applications
M01714 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.429kDa
Background of RAC2
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling RAC2 with M01714 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-RAC2 Antibody (C-term) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Caco2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For RAC2 (Source: Uniprot.org, NCBI)
Gene Name
RAC2
Full Name
Ras-related C3 botulinum toxin substrate 2
Weight
21.429kDa
Superfamily
small GTPase superfamily
Alternative Names
Gx; HSPC022; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP bindingprotein Rac2); rho family, small GTP binding protein Rac2; small GTP-bindingprotein Rac2) RAC2 EN-7, Gx, HSPC022, IMD73A, IMD73B, IMD73C, p21-Rac2 Rac family small GTPase 2 ras-related C3 botulinum toxin substrate 2|Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)|ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)|small G protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAC2, check out the RAC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAC2 Antibody (C-term) (M01714)
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No publications found for M01714
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