Anti-RAD21 Antibody Picoband™

Product Name

Anti-RAD21 Antibody Picoband™

View all RAD21 Antibodies

SKU/Catalog Number

A01864-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RAD21 Antibody Picoband™ catalog # A01864-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RAD21 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01864-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RAD21 recombinant protein (Position: D292-K529).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A01864-2 is reactive to RAD21 in Human, Monkey, Mouse, Rat

Applications

A01864-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

71.69kDa

Background of RAD21

Double-strand-break repair protein rad21 homolog is a protein that in humans is encoded by the RAD21 gene. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RAD21 using anti-RAD21 antibody (A01864-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human Daudi whole cell lysates,
Lane 7: human A431 whole cell lysates,
Lane 8: human T-47D whole cell lysates,
Lane 9: rat brain tissue lysates,
Lane 10: rat pancreas tissue lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 antigen affinity purified polyclonal antibody (Catalog # A01864-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD21 at approximately 72 kDa. The expected band size for RAD21 is at 72 kDa.

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Figure 2. IF analysis of RAD21 using anti-RAD21 antibody (A01864-2).
RAD21 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAD21 Antibody (A01864-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-RAD21 antibody (A01864-2).
Overlay histogram showing HL-60 cells stained with A01864-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD21 Antibody (A01864-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RAD21 (Source: Uniprot.org, NCBI)

Alternative Names

double-strand-break repair protein rad21 homolog; hHR21FLJ25655; HR21; KIAA0078RAD21 (S. pombe) homolog; MCD1; Nuclear matrix protein 1; NXP-1; NXP1HRAD21; protein involved in DNA double-strand break repair; RAD21 homolog (S. pombe); SCC1 homolog; SCC1FLJ40596 RAD21 CDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21 RAD21 cohesin complex component double-strand-break repair protein rad21 homolog|NXP-1|RAD21 homolog|SCC1 homolog|kleisin|nuclear matrix protein 1|protein involved in DNA double-strand break repair|sister chromatid cohesion 1

For more info on RAD21, check out the RAD21 Infographic

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In this infographic, you will see the following information for RAD21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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