Anti-RAIDD CRADD Antibody

Product Name

Anti-RAIDD CRADD Antibody

View all CRADD Antibodies

SKU/Catalog Number

A06509-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RAIDD CRADD Antibody (Catalog # A06509-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

RAIDD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RAIDD CRADD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06509-1)

Host

Rabbit

Contents

RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RAIDD antibody was raised against a 19 amino acid peptide near the center of human RAIDD. The immunogen is located within amino acids 90 - 140 of RAIDD.

Reactive Species

A06509-1 is reactive to CRADD in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

22.7 kDa

Background of CRADD

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1, 3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06509-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 - 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CRADD

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