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Product Info Summary
| SKU: | M04075 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Rak FRK Monoclonal Antibody
SKU/Catalog Number
M04075
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rak FRK Monoclonal Antibody catalog # M04075. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rak FRK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04075)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1A8H2C12
Isotype
IgG
Immunogen
Purified recombinant fragment of Rak (aa2-300) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M04075 is reactive to FRK in Human
Applications
M04075 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.254kDa
Background of FRK
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of FRK using Anti-Rak FRK Monoclonal Antibody (M04075).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast tissues with AEC staining using Rak Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Rak FRK Monoclonal Antibody M04075
Western Blot (WB) analysis using Rak Monoclonal Antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Rak cDNA (2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For FRK (Source: Uniprot.org, NCBI)
Gene Name
FRK
Full Name
Tyrosine-protein kinase FRK
Weight
58.254kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK FRK GTK, PTK5, RAK fyn related Src family tyrosine kinase tyrosine-protein kinase FRK|PTK5 protein tyrosine kinase 5|fyn-related kinase|nuclear tyrosine protein kinase RAK|protein-tyrosine kinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FRK, check out the FRK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FRK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rak FRK Monoclonal Antibody (M04075)
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No publications found for M04075
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