Anti-RanBP1 Antibody Picoband™

Boster Bio Anti-RanBP1 Antibody Picoband™ catalog # A04000-2. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04000-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-RanBP1 Antibody Picoband™

See all RANBP1 products

SKU/Catalog Number

A04000-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RanBP1 Antibody Picoband™ catalog # A04000-2. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RanBP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04000-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human RanBP1 recombinant protein (Position: A2-Q201). Human RanBP1 shares 93.1% amino acid (aa) sequence identity with mouse RanBP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A04000-2 is reactive to RANBP1 in Human, Mouse, Rat

Applications

A04000-2 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For RANBP1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P43487

Gene ID

5902

Gene Name

RANBP1

Full Name

Ran-specific GTPase-activating protein

Weight

23.31kDa

Superfamily

RANBP1 family

Alternative Names

HTF9A; MGC88701; RAN binding protein 1; Ran-binding protein 1; RanBP1; ran-specific GTPase-activating protein RANBP1 HTF9A RAN binding protein 1 ran-specific GTPase-activating protein|HpaII tiny fragments locus 9A|testis secretory sperm-binding protein Li 221n

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RANBP1, check out the RANBP1 Infographic

RANBP1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RANBP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-RanBP1 Antibody Picoband™

Question

My question regarding product A04000-2, anti-RanBP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04000-2 anti-RanBP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-26

Question

We are currently using anti-RanBP1 antibody A04000-2 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

The anti-RanBP1 antibody (A04000-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-28

Question

I was wanting to use your anti-RanBP1 antibody for IHC for rat right testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right testis identification?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

You can see on the product datasheet, A04000-2 anti-RanBP1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-23

Question

I see that the anti-RanBP1 antibody A04000-2 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-23

Question

Does A04000-2 anti-RanBP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Walker

Verified customer

Asked: 2017-10-20

Answer

You can see on the product datasheet, A04000-2 anti-RanBP1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-20

Question

See attached the WB image, lot number and protocol we used for right testis using anti-RanBP1 antibody A04000-2. Please let me know if you require anything else.

Z. Jha

Verified customer

Asked: 2013-01-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-01-24

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A04000-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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