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Product Info Summary
| SKU: | M03448-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-RANBP9 Antibody
SKU/Catalog Number
M03448-1
Size
50 µl
Description
Boster Bio Anti-RANBP9 Antibody (Catalog # M03448-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RANBP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03448-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1815CT202.22.8.37
Isotype
IgG1,k
Immunogen
This RANBP9 antibody is generated from a mouse immunized with a recombinant protein between 1-388 amino acids from the human RANBP9.
Reactive Species
M03448-1 is reactive to RANBP9 in Human
Calculated molecular weight
77.8 kDa
Background of RANBP9
May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03448-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000-1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RANBP9 Antibody at 1:2000-1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: U-2OS whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: CCRF-CEM whole cell lysate
Lane 5: MDA-MB-231whole cell lysate
Lane 6: MOLT-4 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 78 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03448-1 staining RANBP9 in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-RANBP9 Antibody (M03448-1)
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