Anti-RAP80 UIMC1 Antibody

Product Name

Anti-RAP80 UIMC1 Antibody

View all UIMC1 Antibodies

SKU/Catalog Number

A06262

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RAP80 UIMC1 Antibody (Catalog # A06262). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

RAP80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RAP80 UIMC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06262)

Host

Rabbit

Contents

RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80. The immunogen is located within amino acids 520 - 570 of RAP80.

Reactive Species

A06262 is reactive to UIMC1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

79.7 kDa

Background of UIMC1

RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06262 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RAP80 antibody can be used for detection of RAP80 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information UIMC1

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