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Product Info Summary
| SKU: | M00099-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Ras NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099-2
BM4281 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ras NRAS Rabbit Monoclonal Antibody catalog # M00099-2. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBC-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ras
Reactive Species
M00099-2 is reactive to NRAS in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099-2 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IP 1:20
FC 1:50
Positive Control
WB: C6 cell, Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Ras expression in (1) C6 cell lysate; (2) Jurkat cell lysate.
Specific Publications For Anti-Ras NRAS Rabbit Monoclonal Antibody (M00099-2)
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1 Customer Q&As for Anti-Ras NRAS Rabbit Monoclonal Antibody
Question
We are currently using anti-Ras Rabbit Monoclonal antibody M00099-2 for rat tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-Ras Rabbit Monoclonal antibody (M00099-2) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
