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Product Info Summary
| SKU: | M00099 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ras NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099
BM4940 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ras NRAS Rabbit Monoclonal Antibody catalog # M00099. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOFE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ras
Reactive Species
M00099 is reactive to NRAS in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human SH-SY5Y whole cell, human MCF-7 whole cell, human HUVEC whole cell, human U251 whole cell, rat lung tissue , mouse lung tissue, mouse small intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of NRAS using anti-NRAS antibody (M00099).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HUVEC whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAS antigen affinity purified monoclonal antibody (M00099) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRAS at approximately 21 kDa. The expected band size for NRAS is at 21 kDa.
Specific Publications For Anti-Ras NRAS Rabbit Monoclonal Antibody (M00099)
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5 Customer Q&As for Anti-Ras NRAS Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemia using anti-Ras Rabbit Monoclonal antibody M00099. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-04
Question
Would M00099 anti-Ras Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
As indicated on the product datasheet, M00099 anti-Ras Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-Ras Rabbit Monoclonal antibody for IP for rat leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemia identification?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
It shows on the product datasheet, M00099 anti-Ras Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
Do you have a BSA free version of anti-Ras Rabbit Monoclonal antibody M00099 available?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ras Rabbit Monoclonal antibody M00099 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-20
Question
Please see the WB image, lot number and protocol we used for leukemia using anti-Ras Rabbit Monoclonal antibody M00099. Please let me know if you require anything else.
C. Thomas
Verified customer
Asked: 2015-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-03
