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Product Info Summary
| SKU: | A12028 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-RASSF10 Antibody
SKU/Catalog Number
A12028
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RASSF10 Antibody (Catalog # A12028). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
RASSF10 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RASSF10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12028)
Host
Rabbit
Contents
RASSF10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RASSF10 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human RASSF10. The immunogen is located within amino acids 50 - 100 of RASSF10.
Cross-reactivity
RASSF10 antibody will not cross-react with other RAS association domain-containing family members.
Reactive Species
A12028 is reactive to RASSF10 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.9 kDa
Background of RASSF10
RASSF10 is a member of the N-terminal Ras association domain-containing family of proteins, a family of tumor suppressor genes that are frequently epigenetically inactivated in cancer. These proteins consist of RASSF7-10 and are structurally distinct from RASSF1-6, containing the N-terminal Ras association domain but lacking the Sav/RASSF/Hippo protein interaction domains. While little is known of the function of RASSF10, it is inactivated in both childhood leukemia and gliomas. In secondary glioblastomas, RASSF10 methylation can be used as an independent prognostic associated with worst progression-free survival and overall survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12028 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RASSF10 antibody can be used for detection of RASSF10 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RASSF10 in human lung tissue lysate with RASSF10 antibody at 1 μg/mL
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Immunohistochemistry of RASSF10 in human lung tissue with RASSF10 antibody at 5 μg/mL.
Specific Publications For Anti-RASSF10 Antibody (A12028)
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