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Product Info Summary
| SKU: | M00767 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-RBPJK Monoclonal Antibody
SKU/Catalog Number
M00767
BM5578 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RBPJK Monoclonal Antibody catalog # M00767. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBPJK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00767)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEEF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RBPJK Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins.
Reactive Species
M00767 is reactive to RBPJ in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00767 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of RBPJK expression in MCF7 cell lysate.
Specific Publications For Anti-RBPJK Monoclonal Antibody (M00767)
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Customer Q&As
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4 Customer Q&As for Anti-RBPJK Monoclonal Antibody
Question
I was wanting to use your anti-RBPJK Monoclonal antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
As indicated on the product datasheet, M00767 anti-RBPJK Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-07
Question
Will anti-RBPJK Monoclonal antibody M00767 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to the expression profile of placenta, RBPJ is highly expressed in placenta. So, it is likely that anti-RBPJK Monoclonal antibody M00767 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-RBPJK Monoclonal antibody M00767 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
G. Jha
Verified customer
Asked: 2017-03-09
Answer
The anti-RBPJK Monoclonal antibody (M00767) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-09
Question
I see that the anti-RBPJK Monoclonal antibody M00767 works with WB, what is the protocol used to produce the result images on the product page?
A. Gonzalez
Verified customer
Asked: 2015-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-03-20
