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Product Info Summary
| SKU: | M02719-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2)
SKU/Catalog Number
M02719-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2) catalog # M02719-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02719-1)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
7B5D2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human RCC1 recombinant protein (Position: A14-S421).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02719-1 is reactive to RCC1 in Human
Observed Molecular Weight
47 kDa
Calculated molecular weight
45.0 kDa
Background of RCC1
CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02719-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human 293T whole cell, human A431 whole cell, human HepG2 whole cell, human Raji whole cell
Validation Images & Assay Conditions
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Western blot analysis of RCC1 using anti-RCC1 antibody (M02719-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RCC1 antigen affinity purified monoclonal antibody (Catalog # M02719-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 47 kDa. The expected band size for RCC1 is at 47 kDa.
Specific Publications For Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2) (M02719-1)
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