Anti-REEP3 Antibody

Product Name

Anti-REEP3 Antibody

View all Reep3 Antibodies

SKU/Catalog Number

A12243

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-REEP3 Antibody (Catalog # A12243). Tested in ELISA, WB, IHC-P, IF, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

REEP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-REEP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12243)

Host

Rabbit

Contents

REEP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

REEP3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human REEP3. The immunogen is located within the last 50 amino acids of REEP3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

UHRF1BP1 antibody is predicted to not cross-react with UHRF1BP1L

Reactive Species

A12243 is reactive to Reep3 in Human, Mouse, Rat

Applications

A12243 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.214kDa

Background of Reep3

Mammalian odorant receptors require accessory proteins such as RTP1 and RTP2 for functional cell surface expression. Receptor expression-enhancing protein (REEP) family members are transmembrane proteins which interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. Recently studies have shown other chemosensory receptors such as bitter taste receptors are also influenced by RTP and REEP family members. REEP3 has also been suggested as a novel candidate gene for autism and as a loci influencing the plasma levels of the liver enzyme alkaline phosphatase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

REEP3 antibody can be used for detection of REEP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of REEP3 in rat heart tissue lysate with REEP3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of REEP3 in mouse heart tissue with REEP3 antibody at 5 μg/mL.

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Immunofluorescence of REEP3 in mouse heart tissue with REEP3 antibody at 20 μg/mL.

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Immunofluorescence of REEP3 in A431 cells with REEP3 antibody at 20 μg/mL.

Green: REEP3 Antibody (A12243)
Blue: DAPI staining

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Immunocytochemistry of REEP3 in A431 cells with REEP3 antibody at 5 μg/mL.

Gene/Protein Information For Reep3 (Source: Uniprot.org, NCBI)

Alternative Names

C10orf74receptor expression enhancing protein 3; chromosome 10 open reading frame 74; MGC87479; receptor accessory protein 3; receptor expression-enhancing protein 3 Reep3|D10Ucl, D10Ucla1|receptor accessory protein 3|receptor expression-enhancing protein 3

For more info on Reep3, check out the Reep3 Infographic

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In this infographic, you will see the following information for Reep3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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