Anti-REEP3 Antibody Picoband™

Product Name

Anti-REEP3 Antibody Picoband™

View all Reep3 Antibodies

SKU/Catalog Number

A12243-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-REEP3 Antibody Picoband™ catalog # A12243-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-REEP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12243-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human REEP3 , identical to the related mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12243-1 is reactive to Reep3 in Human, Mouse, Rat

Applications

A12243-1 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

29.214kDa

Background of Reep3

Predicted to enable microtubule binding activity. Involved in mitotic nuclear membrane reassembly. Predicted to be integral component of membrane. Predicted to be active in cytoplasmic microtubule; endoplasmic reticulum membrane; and endoplasmic reticulum tubular network.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of REEP3 using anti-REEP3 antibody (A12243-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REEP3 antigen affinity purified polyclonal antibody (Catalog # A12243-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REEP3 at approximately 34 kDa. The expected band size for REEP3 is at 29 kDa.

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Figure 2. IF analysis of REEP3 using anti-REEP3 antibody (A12243-1).
REEP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-REEP3 Antibody (A12243-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Reep3 (Source: Uniprot.org, NCBI)

Alternative Names

C10orf74receptor expression enhancing protein 3; chromosome 10 open reading frame 74; MGC87479; receptor accessory protein 3; receptor expression-enhancing protein 3 Reep3|D10Ucl, D10Ucla1|receptor accessory protein 3|receptor expression-enhancing protein 3

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