Anti-RELB Antibody

Product Name

Anti-RELB Antibody

View all RELB Antibodies

SKU/Catalog Number

M00836-2

Size

50 µl

Description

Boster Bio Anti-RELB Antibody (Catalog # M00836-2). Tested in WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RELB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00836-2)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1684CT450.20.25

Isotype

IgG1,k

Immunogen

This RELB antibody is generated from a mouse immunized with a recombinant protein human RELB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00836-2 is reactive to RELB in Human, Mouse

Applications

M00836-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

62.134kDa

Background of RELB

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF- kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF- kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:5000

Validation Images & Assay Conditions

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Anti-RELB Antibody at 1:5000 dilution + Daudi whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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All lanes : Anti-RELB Antibody at 1:5000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RELB (Source: Uniprot.org, NCBI)

Alternative Names

IREL; I-Rel; nuclear factor of kappalight polypeptide gene enhancer in B-cells 3; RelB; v-rel reticuloendotheliosis viral oncogene homolog B RELB I-REL, IMD53, IREL, REL-B RELB proto-oncogene, NF-kB subunit transcription factor RelB|v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)|v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

For more info on RELB, check out the RELB Infographic

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In this infographic, you will see the following information for RELB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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