Click image to see more details
Product Info Summary
| SKU: | PB9222 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MUM1/IRF4 Antibody Picoband®
SKU/Catalog Number
PB9222
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUM1/IRF4 Antibody Picoband® catalog # PB9222. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUM1/IRF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9222)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9222 is reactive to IRF4 in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
51.8 kDa
Background of IRF4
Interferon regulatory factor 4 (IRF4), also known as MUM1, is a protein that in humans is encoded by the IRF4 gene. It is located on 6p25.3. IRF4 is a transcription factor, and it is essential for the development of T helper-2 (Th2) cells, IL17 -producing Th17 cells, and IL9 -producing Th9 cells. In melanocytic cells, the IRF4 gene may be regulated by MITF. IRF4 is a transcription factor that has been implicated in acute leukemia. This gene is strongly associated with pigmentation, sensitivity of skin to sun exposure, freckles, blue eyes, and brown hair color. What's more, IRF4 inhibition is toxic to myeloma cell lines, regardless of transforming oncogenic mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9222 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-MUM1 antibody, PB9222, Western blotting
All lanes: Anti MUM1 (PB9222) at 0.5ug/ml
WB: Recombinant Human MUM1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Click image to see more details
Anti-MUM1 antibody, PB9222, Western blotting
All lanes: Anti MUM1 (PB9222) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 40KD
Click image to see more details
Anti-MUM1 antibody, PB9222, IHC(P)
IHC(P): Human Tonsil Tissue
Specific Publications For Anti-MUM1/IRF4 Antibody Picoband® (PB9222)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MUM1/IRF4 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-MUM1/IRF4 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-MUM1/IRF4 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MUM1/IRF4 antibody (PB9222)?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-MUM1/IRF4 antibody (PB9222). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
I would like to test anti-MUM1/IRF4 antibody PB9222 on human spleen for research purposes, then I may be interested in using anti-MUM1/IRF4 antibody PB9222 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-MUM1/IRF4 antibody PB9222, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
We were happy with the WB result of your anti-MUM1/IRF4 antibody. However we have seen positive staining in spleen nucleus. using this antibody. Is that expected? Could you tell me where is IRF4 supposed to be expressed?
Z. Krishna
Verified customer
Asked: 2019-11-04
Answer
From literature, spleen does express IRF4. Generally IRF4 expresses in nucleus. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2019-11-04
Question
I see that the anti-MUM1/IRF4 antibody PB9222 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-06
Question
Is there a BSA free version of anti-MUM1/IRF4 antibody PB9222 available?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUM1/IRF4 antibody PB9222 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-05
Question
We bought anti-MUM1/IRF4 antibody for WB on lymph node in a previous experiment. I am using human, and We are going to use the antibody for IHC next. My lab would like examining lymph node as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
W. Krishna
Verified customer
Asked: 2019-08-01
Answer
I have checked the website and datasheets of our anti-MUM1/IRF4 antibody and I see that PB9222 has been validated on human in both WB and IHC. Thus PB9222 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-01
Question
I have attached the WB image, lot number and protocol we used for spleen using anti-MUM1/IRF4 antibody PB9222. Please let me know if you require anything else.
E. Parker
Verified customer
Asked: 2019-07-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-MUM1/IRF4 antibody PB9222. Let me know if you need anything else.
V. Singh
Verified customer
Asked: 2018-11-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-21
Question
Will anti-MUM1/IRF4 antibody PB9222 work for IHC with spleen?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
According to the expression profile of spleen, IRF4 is highly expressed in spleen. So, it is likely that anti-MUM1/IRF4 antibody PB9222 will work for IHC with spleen.
Boster Scientific Support
Answered: 2018-10-03
Question
I was wanting to use your anti-MUM1/IRF4 antibody for IHC for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
You can see on the product datasheet, PB9222 anti-MUM1/IRF4 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-06
Question
Will PB9222 anti-MUM1/IRF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Yang
Verified customer
Asked: 2017-02-20
Answer
It shows on the product datasheet, PB9222 anti-MUM1/IRF4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-20
Question
Is this PB9222 anti-MUM1/IRF4 antibody reactive to the isotypes of IRF4?
L. Krishna
Verified customer
Asked: 2016-05-19
Answer
The immunogen of PB9222 anti-MUM1/IRF4 antibody is E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-19
Question
you antibody using your anti-MUM1/IRF4 antibody for interferon-gamma-mediated signaling pathway studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
G. Baker
Verified customer
Asked: 2015-01-21
Answer
We appreciate your inquiry. This PB9222 anti-MUM1/IRF4 antibody is tested on human tonsil tissue, hela whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-21
Question
We are currently using anti-MUM1/IRF4 antibody PB9222 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
F. Krishna
Verified customer
Asked: 2014-10-03
Answer
The anti-MUM1/IRF4 antibody (PB9222) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-03
Question
I have a question about product PB9222, anti-MUM1/IRF4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Rodriguez
Verified customer
Asked: 2013-11-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9222 anti-MUM1/IRF4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-15
Question
We have observed staining in human spleen. What should we do? Is anti-MUM1/IRF4 antibody supposed to stain spleen positively?
E. Anderson
Verified customer
Asked: 2013-09-13
Answer
From literature spleen does express IRF4. From Uniprot.org, IRF4 is expressed in endocervix, spleen, skin, lymph node, among other tissues. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2013-09-13
