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- Table of Contents
IRF4 antibody (PB9222) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-MUM1 antibody, PB9222, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
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Anti-MUM1/IRF4 Antibody Picoband®
IRF4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
IRF4 antibody was positively detected by WB in:
HELA Whole Cell, JURKAT Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IRF4 antibody was positively detected by IHC in:
Human Tonsil Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Product Information
Product Name
Anti-MUM1/IRF4 Antibody Picoband®
View all IRF4 Antibodies
View all IRF4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
51.8 kDa
Background of IRF4
Interferon regulatory factor 4 (IRF4), also known as MUM1, is a protein that in humans is encoded by the IRF4 gene. It is located on 6p25.3. IRF4 is a transcription factor, and it is essential for the development of T helper-2 (Th2) cells, IL17 -producing Th17 cells, and IL9 -producing Th9 cells. In melanocytic cells, the IRF4 gene may be regulated by MITF. IRF4 is a transcription factor that has been implicated in acute leukemia. This gene is strongly associated with pigmentation, sensitivity of skin to sun exposure, freckles, blue eyes, and brown hair color. What's more, IRF4 inhibition is toxic to myeloma cell lines, regardless of transforming oncogenic mechanism.
Cite This Product
Anti-MUM1/IRF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9222)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
97%
High Homology
Rabbit
97%
High Homology
Cow
93%
High Homology
Mouse
92%
High Homology
Rat
92%
High Homology
Sheep
72%
Moderate Homology
Zebrafish
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IRF4 antibody
Immunohistochemistry (IHC) protocol for IRF4 antibody
Publications
Application:
Species:
TIPE2, a negative regulator of TLR signaling, regulates p27 through IRF4-induced signaling
Published: 2016-01-14
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.49
Species: Human
PMID: 26781452
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Product Promise
We promise Anti-MUM1/IRF4 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9222
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026