Anti-Retinal S antigen/SAG Antibody Picoband™

Product Name

Anti-Retinal S antigen/SAG Antibody Picoband™

View all S-arrestin Antibodies

SKU/Catalog Number

A03775

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Retinal S antigen/SAG Antibody Picoband™ catalog # A03775. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Retinal S antigen/SAG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03775)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Retinal S antigen recombinant protein (Position: R193-E405).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03775 is reactive to SAG in Human, Mouse, Rat

Applications

A03775 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

45-55 kDa

Calculated molecular weight

45.12kDa

Background of S-arrestin

S-arrestin is a protein that in humans is encoded by the SAG gene. Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Retinal S antigen using anti-Retinal S antigen antibody (A03775).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat eye ball tissue lysates,
Lane 2: mouse eye ball tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Retinal S antigen antigen affinity purified polyclonal antibody (Catalog # A03775) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Retinal S antigen at approximately 45-55KD. The expected band size for Retinal S antigen is at 45KD.

Gene/Protein Information For SAG (Source: Uniprot.org, NCBI)

Alternative Names

arrestin 1; RP47,48 kDa protein; S-antigen; retina and pineal gland (arrestin); S-arrestin; visual arrestin SAG RP47, S-AG S-antigen visual arrestin S-arrestin|48 kDa protein|S-antigen; retina and pineal gland (arrestin)|arrestin 1|retinal S-antigen (48 KDa protein)|rod arrestin|rod photoreceptor arrestin

For more info on SAG, check out the SAG Infographic

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In this infographic, you will see the following information for SAG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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