Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband™ (monoclonal, 5E7)

Product Name

Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband™ (monoclonal, 5E7)

View all RXR alpha/NR2B1 Antibodies

SKU/Catalog Number

M01299

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband™ (monoclonal, 5E7) catalog # M01299. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband™ (monoclonal, 5E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01299)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Monoclonal

Clone Number

5E7

Isotype

Mouse IgG1

Immunogen

E. coli-derived human RXRA recombinant protein (Position: A226-T462).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01299 is reactive to RXRA in Human, Mouse, Rat

Applications

M01299 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

50.811kDa

Background of RXR alpha/NR2B1

Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Retinoid X Receptor alpha/RXRA using anti-Retinoid X Receptor alpha/RXRA antibody (M01299).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates;
Lane 2: human A549 whole cell lysates;
Lane 3: human HepG2 whole cell lysates;
Lane 4: human Caco-2 whole cell lysates;
Lane 5: human HL-60 whole cell lysates;
Lane 6: rat liver tissue lysates;
Lane 7: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Retinoid X Receptor alpha/RXRA antigen affinity purified monoclonal antibody (Catalog # M01299) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Retinoid X Receptor alpha/RXRA at approximately 55KD. The expected band size for Retinoid X Receptor alpha/RXRA is at 51KD.

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Figure 2. Flow Cytometry analysis of A549 cells using anti-Retinoid X Receptor alpha/RXRA antibody (M01299).
Overlay histogram showing A549 cells stained with M01299 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-Retinoid X Receptor alpha/RXRA antibody (M01299).
Overlay histogram showing CACO-2 cells stained with M01299 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RXRA (Source: Uniprot.org, NCBI)

Alternative Names

FLJ00280; FLJ00318; FLJ16020; MGC102720; NR2B1; NR2B1FLJ16733; Nuclear receptor subfamily 2 group B member 1; retinoic acid receptor RXR-alpha; Retinoid X receptor alpha; retinoid X receptor, alpha; RXR alpha; RXRA RXRA NR2B1 retinoid X receptor alpha retinoic acid receptor RXR-alpha|nuclear receptor subfamily 2 group B member 1|retinoid X nuclear receptor alpha

For more info on RXRA, check out the RXRA Infographic

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In this infographic, you will see the following information for RXRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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