Anti-ZIP1 SLC39A1 Antibody

Product Name

Anti-ZIP1 SLC39A1 Antibody

View all SLC39A1 Antibodies

SKU/Catalog Number

A03886

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZIP1 SLC39A1 Antibody (Catalog # A03886). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ZIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZIP1 SLC39A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03886)

Host

Rabbit

Contents

ZIP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1. The immunogen is located within amino acids 40 - 90 of ZIP1.

Cross-reactivity

At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.

Reactive Species

A03886 is reactive to SLC39A1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

34.3 kDa

Background of SLC39A1

ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03886 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SLC39A1

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