Click image to see more details
Product Info Summary
| SKU: | M03820 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Goat, Human, Monkey, Mouse, Rabbit, Rat, Chimpanzee |
| Host: | Mouse |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody
SKU/Catalog Number
M03820
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody (Catalog # M03820). Tested in IHC applications. This antibody reacts with Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03820)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: G4E4
Isotype
IgG1, kappa
Immunogen
Retinol binding protein-1 Purified from human plasma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03820 is reactive to in Goat, Human, Monkey, Mouse, Rabbit, Rat, Chimpanzee
Applications
M03820 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
23010 MW
Background of
This monoclonal antibody recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Anti-RBP1 Mouse Monoclonal Antibody (G4E4).
Specific Publications For Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody (M03820)
Hello CJ!
No publications found for M03820
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody
Question
I see that the anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody M03820 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-31
Question
I have a question about product M03820, anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03820 anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody M03820. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
Will anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody M03820 work on monkey IHC with fetal liver?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Our lab technicians have not validated anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody M03820 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Retinol Binding Protein-1 (RBP1) Monoclonal antibody M03820 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey fetal liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-11
