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Product Info Summary
| SKU: | A11393 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RETSAT Antibody Picoband®
SKU/Catalog Number
A11393
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RETSAT Antibody Picoband® catalog # A11393. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RETSAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11393)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RETSAT recombinant protein (Position: E35-K597).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11393 is reactive to RETSAT in Human, Rat
Observed Molecular Weight
67 kDa
Calculated molecular weight
66.8 kDa
Background of RETSAT
In enzymology, an all-trans-retinol 13,14-reductase is an enzyme, encoded by the RETSAT gene. Predicted to enable all-trans-retinol 13,14-reductase activity. Predicted to be involved in retinol metabolic process. Located in membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11393 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Rat |
| Flow Cytometry (Fixed) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of RETSAT using anti-RETSAT antibody (A11393).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HUH-7 whole cell lysates,
Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 4: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RETSAT antigen affinity purified polyclonal antibody (Catalog # A11393) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RETSAT at approximately 67 kDa. The expected band size for RETSAT is at 67 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-RETSAT antibody (A11393).
Overlay histogram showing HepG2 cells stained with A11393 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RETSAT Antibody (A11393, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-RETSAT Antibody Picoband® (A11393)
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