Anti-RGS21 Antibody

Product Name

Anti-RGS21 Antibody

View all RGS21 Antibodies

SKU/Catalog Number

A17313

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RGS21 Antibody (Catalog # A17313). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

RGS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RGS21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17313)

Host

Rabbit

Contents

RGS21 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21. The immunogen is located within amino acids 40 - 90 of RGS21.

Reactive Species

A17313 is reactive to RGS21 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

17.7 kDa

Background of RGS21

Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A17313 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RGS21

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