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Product Info Summary
| SKU: | A14223 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-RHBDD3 Antibody
SKU/Catalog Number
A14223
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RHBDD3 Antibody (Catalog # A14223). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RHBDD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RHBDD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14223)
Host
Rabbit
Contents
RHBDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RHBDD3 antibody was raised against a 13 amino acid synthetic peptide from near the center of human RHBDD3. The immunogen is located within amino acids 170 - 220 of RHBDD3.
Reactive Species
A14223 is reactive to RHBDD3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
40.5 kDa
Background of RHBDD3
The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD3 was initially identified as a novel pituitary tumor apoptosis gene (PTAG) whose expression was reduced in certain pituitary adenomas. Overexpression of RHBDD3 in AtT20 cells showed an increase in apoptotic activity and caspase activation in response to bromocriptine, and apoptosis-inducing dopamine D2 analog, suggesting that reactivation of RHBDD3 in tumors may be a therapeutically useful tool. Later experiments also showed that RHBDD3 expression is also reduced in several primary colorectal tumors, indicating that loss of RHBDD3 contributes to a blunted apoptotic response and probably predisposes cells towards malignant transformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14223 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RHBDD3 antibody can be used for detection of RHBDD3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RHBDD3 in rat lung tissue lysate with RHBDD3 antibody at 1 μg/mL.
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Immunofluorescence of RHBDD32 in rat lung tissue with RHBDD3 antibody at 20 μg/mL.
Specific Publications For Anti-RHBDD3 Antibody (A14223)
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