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- Table of Contents
RHOA antibody (M00207) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Immunofluorescent analysis of Jurkat cells, using Rho A Antibody.
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Anti-Rho A Rabbit Monoclonal Antibody
RHOA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
RHOA antibody was positively detected by WB in:
human Hela whole cell, human U251 whole cell, human SIHA whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RHOA antibody was positively detected by ICC/IF in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
FC
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Rho A Rabbit Monoclonal Antibody
View all RHOA Antibodies
View all RHOA Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FAC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rho A
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
21.8 kDa
Cite This Product
Anti-Rho A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207)
Protocols
Western Blot protocol for RHOA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RHOA antibody
Publications
Application:
Species:
In vitro axotomy models regulated by ROCK inhibitor for studying neuroregeneration and growth cone morphology
Published: 2025-08-23
Journal: Biomedical Journal
Application: WB
Impact Factor: 4.40
Species: Rat
PMID: 40854379
Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway
Published: 2024-01-11
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.30
Species: Rat
PMID: 38212902
AKBA Promotes Axonal Regeneration via RhoA/Rictor to Repair Damaged Sciatic Nerve
Published: 2022-12-14
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB,IF
Impact Factor: 6.21
Species: Rat
PMID: 36555556
High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway
Published: 2022-07-08
Journal: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Application: WB
Impact Factor: 4.93
Species: Mouse
PMID: 35881407
Dual efficacy of Fasudil at improvement of survival and reinnervation of flap through RhoA/ROCK/PI3K/Akt pathway
Published: 2022-03-22
Journal: International Wound Journal
Application: WB
Impact Factor: 3.10
Species: Mouse
PMID: 35315211
Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling
Published: 2020-05-09
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: WB
Impact Factor: 2.84
Species: Rat
PMID: 32386193
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction
Published: 2019-04-17
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 3.74
Species: Human
PMID: 31004838
Macrophage differentiation induced by PMA is mediated by activation of RhoA/ROCK signaling
Published: 2017-11-16
Journal: JOURNAL OF TOXICOLOGICAL SCIENCES
Application: WB
Impact Factor: 1.44
Species: Human
PMID: 29142175
The Effect of Butylphthalide on the Brain Edema, Blood–Brain Barrier of Rats After Focal Cerebral Infarction and the Expression of Rho A
Published: 2014-01-18
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: IHC
Impact Factor: 2.38
Species: Rat
PMID: 24442989
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Product Promise
We promise Anti-Rho A Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00207
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026