Anti-Rhomboid-related protein 4 RHBDD1 Antibody

Product Name

Anti-Rhomboid-related protein 4 RHBDD1 Antibody

View all RHBDD1 Antibodies

SKU/Catalog Number

A09636

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Rhomboid-related protein 4 RHBDD1 Antibody (Catalog # A09636). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

RHBDD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Rhomboid-related protein 4 RHBDD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09636)

Host

Rabbit

Contents

RHBDD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RHBDD1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1. The immunogen is located within the last 50 amino acids of RHBDD1.

Reactive Species

A09636 is reactive to RHBDD1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

35.8 kDa

Background of RHBDD1

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09636 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RHBDD1

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