Product Info Summary
SKU: | A10323-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Ribosomal Protein L28 RPL28 Antibody
SKU/Catalog Number
A10323-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ribosomal Protein L28 RPL28 Antibody catalog # A10323-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ribosomal Protein L28 RPL28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10323-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Ribosomal Protein L28
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10323-1 is reactive to RPL28 in Human, Mouse, Rat
Applications
A10323-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
15.748kDa
Background of RPL28
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Frigerio J.-M., Biochim. Biophys. Acta 1262:64-68(1995).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Ribosomal Protein L28 RPL28 Antibody A10323-1
Western Blot (WB) analysis of specific cells using Ribosomal Protein L28 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RPL28 (Source: Uniprot.org, NCBI)
Gene Name
RPL28
Full Name
60S ribosomal protein L28
Weight
15.748kDa
Superfamily
eukaryotic ribosomal protein eL28 family
Alternative Names
FLJ43307,60S ribosomal protein L28; ribosomal protein L28 RPL28 L28 ribosomal protein L28 60S ribosomal protein L28|large ribosomal subunit protein eL28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL28, check out the RPL28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ribosomal Protein L28 RPL28 Antibody (A10323-1)
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No publications found for A10323-1
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