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Product Info Summary
| SKU: | A06025 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat, Cat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Ribosomal Protein L7 RPL7 Antibody
SKU/Catalog Number
A06025
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ribosomal Protein L7 RPL7 Antibody catalog # A06025. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Cat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ribosomal Protein L7 RPL7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06025)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPL7. AA range:199-248
Reactive Species
A06025 is reactive to RPL7 in Human, Mouse, Rat, Cat
Observed Molecular Weight
39 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06025 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Ribosomal Protein L7 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of customer's (cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000
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Western blot analysis of lysates from 293 and HeLa cells, using RPL7 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using RPL7 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Ribosomal Protein L7 RPL7 Antibody (A06025)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Ribosomal Protein L7 RPL7 Antibody
Question
Would anti-Ribosomal Protein L7 antibody A06025 work on goat WB with eye?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Our lab technicians have not tested anti-Ribosomal Protein L7 antibody A06025 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Ribosomal Protein L7 antibody A06025 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat eye in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is this A06025 anti-Ribosomal Protein L7 antibody reactive to the isotypes of RPL7?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The immunogen of A06025 anti-Ribosomal Protein L7 antibody is Synthesized peptide derived from the C-terminal region of human Ribosomal Protein L7. at AA rangle: 180-260. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-28
Question
We are currently using anti-Ribosomal Protein L7 antibody A06025 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
S. Rodriguez
Verified customer
Asked: 2019-04-02
Answer
The anti-Ribosomal Protein L7 antibody (A06025) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-02
