Product Info Summary
SKU: | M03195 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Rictor Monoclonal Antibody
SKU/Catalog Number
M03195
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rictor Monoclonal Antibody catalog # M03195. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rictor Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03195)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
7B3
Isotype
IgG
Immunogen
Purified recombinant fragment of human Rictor expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03195 is reactive to Rictor in Human, Monkey, Mouse
Applications
M03195 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
191.57kDa
Background of Rictor
Anti-Procalcitonin antibody detects human Procalcitonin. Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, a N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. Flow Cytometry validation of RICTOR using Anti-Rictor Monoclonal Antibody (M03195).
Flow cytometric (FCM) analysis of HeLa cells using Rictor Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 3. Immunofluorescent staining data of RICTOR using Anti-Rictor Monoclonal Antibody (M03195).
Immunofluorescence (IF) analysis of NIH/3T3 cells using Rictor Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of RICTOR using Anti-Rictor Monoclonal Antibody (M03195).
Immunohistochemistry (IHC) analysis of paraffin-embedded thyroid gland tissues (left) and Human Breast Carcinoma (right) with DAB staining using Rictor Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Rictor Monoclonal Antibody M03195
Western Blot (WB) analysis using Rictor Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For Rictor (Source: Uniprot.org, NCBI)
Gene Name
Rictor
Full Name
Rapamycin-insensitive companion of mTOR
Weight
191.57kDa
Superfamily
RICTOR family
Alternative Names
AVO3 homolog; AVO3; hAVO3; KIAA1999DKFZp686B11164; mAVO3; MGC39830; PIA; Pianissimo; rapamycin-insensitive companion of mTOR; Rictor; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3 Rictor|4921505C17Rik, 6030405M08Rik, AVO3, AW492497, D530039E11Rik|RPTOR independent companion of MTOR, complex 2|rapamycin-insensitive companion of mTOR|AVO3 homolog|mAVO3|protein pianissimo
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rictor, check out the Rictor Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rictor: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rictor Monoclonal Antibody (M03195)
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No publications found for M03195
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Customer Reviews
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Customer Q&As
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