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Product Info Summary
| SKU: | RP1095 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RIP140/NRIP1 Antibody
SKU/Catalog Number
RP1095
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIP140/NRIP1 Antibody catalog # RP1095. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP140/NRIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1095)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RIP140, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1095 is reactive to NRIP1 in Human, Mouse, Rat
Applications
RP1095 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
126.942kDa
Background of RIP140
Nuclear receptor-interacting protein 1 (NRIP1), also known as receptor-interacting protein 140 (RIP140), is a protein that in humans is encoded by the NRIP1 gene. It is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein is a key regulator which modulates transcriptional activity of a variety of transcription factors, including the estrogen receptor. RIP140 has an important role in regulating lipid and glucose metabolism, and regulates gene expression in metabolic tissues including heart, skeletal muscle, and liver. A major role for RIP140 in adipose tissue is to block the expression of genes involved in energy dissipation and mitochondrial uncoupling, including uncoupling protein 1 and carnitine palmitoyltransferase 1b.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
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Anti-RIP140 antibody, RP1095, Western blotting
All lanes: Anti RIP140 (RP1095) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 127KD
Observed bind size: 150KD
Protein Target Info & Infographic
Gene/Protein Information For NRIP1 (Source: Uniprot.org, NCBI)
Gene Name
NRIP1
Full Name
Nuclear receptor-interacting protein 1
Weight
126.942kDa
Alternative Names
10RIP140Receptor-interacting protein 140; FLJ77253; nuclear receptor interacting protein 1; nuclear receptor-interacting protein 1; receptor interacting protein 140 NRIP1 CAKUT3, RIP140 nuclear receptor interacting protein 1 nuclear receptor-interacting protein 1|nuclear factor RIP140|receptor-interacting protein 140
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRIP1, check out the NRIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIP140/NRIP1 Antibody (RP1095)
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No publications found for RP1095
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-RIP140/NRIP1 Antibody
Question
We are currently using anti-RIP140/NRIP1 antibody RP1095 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
J. Yang
Verified customer
Asked: 2015-01-16
Answer
The anti-RIP140/NRIP1 antibody (RP1095) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-16
