Click image to see more details
Product Info Summary
| SKU: | M00202-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03)
SKU/Catalog Number
M00202-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03) catalog # M00202-3. Tested in WB, ICC/IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00202-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
35R03
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser232 of Human RIP3.
Reactive Species
M00202-3 is reactive to Ripk3 in Mouse, Rat
Observed Molecular Weight
57 kDa
Calculated molecular weight
53.3 kDa
Background of Ripk3
Receptor-interacting serine/threonine-protein kinase 3(RIPK3), also known as RIP3 is an enzyme that in humans is encoded by the RIPK3 gene. This gene is mapped to 14q12. The product of this gene is a member of the receptor-interacting protein(RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor(TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00202-3 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-200
Positive Control
WB: human Jurkat whole cell lysates, rat heart tissue lysates, mouse heart tissue lysates
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RIP3/RIPK3 using anti-RIP3/RIPK3 antibody (M00202-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP3/RIPK3 antigen affinity purified monoclonal antibody (M00202-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RIP3/RIPK3 at approximately 57 kDa. The expected band size for RIP3/RIPK3 is at 57 kDa.
Specific Publications For Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03) (M00202-3)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
