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Product Info Summary
| SKU: | M00202 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-RIPK3 Antibody
SKU/Catalog Number
M00202
Size
50 µl
Description
Boster Bio Anti-RIPK3 Antibody (Catalog # M00202). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RIPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00202)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2013CT892.86.49
Isotype
IgG1,κ
Immunogen
This RIPK3 antibody is generated from a mouse immunized with a recombinate protein from the human region of human RIPK3.
Reactive Species
M00202 is reactive to RIPK3 in Human
Calculated molecular weight
56.9 kDa
Background of RIPK3
Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00202 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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All lanes : Anti-RIPK3 Antibody at 1:1000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: SW620 whole cell lysate
Lane 4: THP-1 whole cell lysate
Lane 5: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-RIPK3 Antibody (M00202)
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