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Product Info Summary
| SKU: | A03457 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RNF20 Antibody
SKU/Catalog Number
A03457
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RNF20 Antibody (Catalog # A03457). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RNF20 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-RNF20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03457)
Host
Rabbit
Contents
RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20. The immunogen is located within amino acids 130 - 180 of RNF20.
Cross-reactivity
Multiple isoforms of RNF20 are known to exist.
Reactive Species
A03457 is reactive to RNF20 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
113.7 kDa
Background of RNF20
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03457 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 μg/ml.
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Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.
Specific Publications For Anti-RNF20 Antibody (A03457)
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