This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
RNF20 Antibodies
AND RNF20 ELISA kits, RNF20 Proteins
Many biological assays use antibodies to detect RNF20, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNF20 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNF20 antibodies...
Designing a Western blot for RNF20? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the RNF20 Western Blot GuideRNF20 Infographic by Boster Bio
All RNF20 Products
Product
Figures
Specificity
Applications
Price
Human, Rat
ELISA, Flow Cytometry, IF, ICC, WB
$370
Cat # A03457-1
100 μg/vial
Cat # A03457-1
100 μg/vial
Human, Mouse
Flow Cytometry, IP, IF, ICC, WB
$370
Cat # M03457-1
100 μl
Cat # M03457-1
100 μl
Human, Mouse, Rat
WB
$370
Cat # M03457-4
100 μg/vial
Cat # M03457-4
100 μg/vial
Human, Mouse
Flow Cytometry, IP, IF, ICC, WB
$370
Cat # M03457
100 μl
Cat # M03457
100 μl
Human, Mouse
ELISA
$540
Cat # EKC2624
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2624
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse
ELISA
$480
Cat # EKC1504
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1504
1 kit, containing one 96-well plate and all necessary reagents
RNF20 Overview
Facts about E3 ubiquitin-protein ligase BRE1A.
E3 ubiquitin-protein ligase BRE1A (RNF20)
Part of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively).
It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory.
Gene Information
| Human | |
|---|---|
| Gene Name: | RNF20 |
| Uniprot: | Q5VTR2 |
| Entrez: | 56254 |
Family
| Belongs to: |
|---|
| BRE1 family |
Alternative Names
BRE1 E3 ubiquitin ligase homolog; BRE1; BRE1-A; BRE1AMGC129667; E3 ubiquitin-protein ligase BRE1A; EC 6.3.2; EC 6.3.2.-; FLJ11189; FLJ20382; hBRE1MGC129668; homolog of S. cerevisiae BRE1; KAIA2779; ring finger protein 20KIAA2779
Molecular Weight
Mass (kDA):
113.662 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 9q31.1 |
| Sequence: | 9; NC_000009.12 (101533853..101563344) |
Tissue Specificity
Expressed in the normal brain and also in malignant gliomas (at protein level).
Subcellular Localizations
Nucleus.
Diseases
- Neoplasms
- Malignant Neoplasms
- Genomic Instability
- Carcinoma
- Multiple Endocrine Neoplasia Type 1
- Colorectal Neoplasms
- Instabilities, Chromosomal
- Exposure To Ionizing Radiation
- Seminoma
- Von Hippel-lindau Syndrome
- Glioma
- Testicular Seminoma
Interacting Proteins
- CHEK2
- COPS5
- HTT
- RNF40
- UBE2E1
- USHBP1
Pathways
- Methylation
- Histone Modification
- Cell Cycle
- Chromatin Organization
- Cell Growth
- Cell Migration
- Cell Cycle Checkpoint
- Gene Silencing
- Stem Cell Differentiation
- Cellular Senescence
- Histone Monoubiquitination
- Immune Response
- Histone Mrna 3'-end Processing
PTMs
- Methylation
- Ubiquitination
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF20
bosterbio.com/bosterbio-gene-info-cards/RNF20
References
PMID: 16307923 by Zhu B., et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation.
PMID: 16337599 by Kim J., et al. The human homolog of yeast BRE1 functions as a transcriptional coactivator through direct activator interactions.