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- Table of Contents
RNF20 antibody (M03457-2) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
All lanes : Anti-RNF20 Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: U87-MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 114 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: U87-MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 114 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-RNF20 Antibody
RNF20 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-RNF20 Antibody
View all RNF20 Antibodies
View all RNF20 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1594CT552.128.36
Isotype
IgG1,k
Immunogen
This RNF20 antibody is generated from a mouse immunized with a recombinant protein of human RNF20.
Calculated Molecular Weight
113.7 kDa
Background of RNF20
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Cite This Product
Anti-RNF20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03457-2)
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Product Promise
We promise Anti-RNF20 Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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M03457-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026