Anti-RNF20 Antibody Picoband™ (monoclonal, 3C6E2)

Product Name

Anti-RNF20 Antibody Picoband™ (monoclonal, 3C6E2)

View all RNF20 Antibodies

SKU/Catalog Number

M03457-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF20 Antibody Picoband™ (monoclonal, 3C6E2) catalog # M03457-4. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RNF20 Antibody Picoband™ (monoclonal, 3C6E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03457-4)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3C6E2

Isotype

IgG2b

Immunogen

E.coli-derived human RNF20 recombinant protein (Position: E123-S389).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M03457-4 is reactive to RNF20 in Human, Mouse, Rat

Applications

M03457-4 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

114 kDa

Calculated molecular weight

113.662kDa

Background of RNF20

E3 ubiquitin-protein ligase BRE1A is an enzyme that in humans is encoded by the RNF20 gene. The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RNF20 using anti-RNF20 antibody (M03457-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RNF20 antigen affinity purified monoclonal antibody (Catalog # M03457-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RNF20 at approximately 114 kDa. The expected band size for RNF20 is at 114 kDa.

Gene/Protein Information For RNF20 (Source: Uniprot.org, NCBI)

Alternative Names

BRE1 E3 ubiquitin ligase homolog; BRE1; BRE1-A; BRE1AMGC129667; E3 ubiquitin-protein ligase BRE1A; EC 6.3.2; EC 6.3.2.-; FLJ11189; FLJ20382; hBRE1MGC129668; homolog of S. cerevisiae BRE1; KAIA2779; ring finger protein 20KIAA2779 RNF20 BRE1, BRE1A, hBRE1 ring finger protein 20 E3 ubiquitin-protein ligase BRE1A|BRE1 E3 ubiquitin ligase homolog|RING-type E3 ubiquitin transferase BRE1A|homolog of S. cerevisiae BRE1

For more info on RNF20, check out the RNF20 Infographic

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In this infographic, you will see the following information for RNF20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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