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Product Info Summary
| SKU: | M03457-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-RNF20 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03457-1
BM3915 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RNF20 Rabbit Monoclonal Antibody catalog # M03457-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF20 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03457-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RNF20
Reactive Species
M03457-1 is reactive to RNF20 in Human, Mouse
Observed Molecular Weight
114 kDa
Calculated molecular weight
113.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03457-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human A549 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of RNF20 using anti-RNF20 antibody (M03457-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 antigen affinity purified monoclonal antibody (M03457-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RNF20 at approximately 114 kDa. The expected band size for RNF20 is at 114 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-RNF20 Rabbit Monoclonal Antibody (M03457-1)
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5 Customer Q&As for Anti-RNF20 Rabbit Monoclonal Antibody
Question
Would anti-RNF20 Rabbit Monoclonal antibody M03457-1 work on canine IP with amygdala melanoma?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Our lab technicians have not validated anti-RNF20 Rabbit Monoclonal antibody M03457-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-RNF20 Rabbit Monoclonal antibody M03457-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine amygdala melanoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-30
Question
Is a blocking peptide available for product anti-RNF20 Rabbit Monoclonal antibody (M03457-1)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We do provide the blocking peptide for product anti-RNF20 Rabbit Monoclonal antibody (M03457-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this M03457-1 anti-RNF20 Rabbit Monoclonal antibody reactive to the isotypes of RNF20?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The immunogen of M03457-1 anti-RNF20 Rabbit Monoclonal antibody is A synthesized peptide derived from human RNF20. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-RNF20 Rabbit Monoclonal antibody M03457-1 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
V. Jones
Verified customer
Asked: 2013-11-18
Answer
The anti-RNF20 Rabbit Monoclonal antibody (M03457-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-18
Question
Will M03457-1 anti-RNF20 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Patel
Verified customer
Asked: 2013-06-17
Answer
It shows on the product datasheet, M03457-1 anti-RNF20 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-17
