Anti-RNF34 Antibody

Product Name

Anti-RNF34 Antibody

View all RNF34 Antibodies

SKU/Catalog Number

A08108

Size

500ug

Form

Lyophilized

Description

Boster Bio Anti-RNF34 Antibody (Catalog # A08108). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-RNF34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08108)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

A08108 is reactive to RNF34 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

41.6 kDa

Background of RNF34

RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08108 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:20,000
IF Microscopy: User optimized
WB: 1:500 - 1:3,000
This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.

Validation Images & Assay Conditions

See full target information RNF34

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