Anti-RNF34 Antibody

Product Name

Anti-RNF34 Antibody

View all RNF34 Antibodies

SKU/Catalog Number

A08108

Size

500ug

Form

Lyophilized

Description

Boster Bio Anti-RNF34 Antibody (Catalog # A08108). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-RNF34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08108)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

EM-11

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

A08108 is reactive to RNF34 in Human

Applications

A08108 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

41.641kDa

Background of RNF34

RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 500 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:20,000
IF Microscopy: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RNF34 using anti-RNF34 antibody (A08108).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (Catalog # A08108) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RNF34.

Gene/Protein Information For RNF34 (Source: Uniprot.org, NCBI)

Alternative Names

CARP1; Caspase regulator CARP1; Caspases-8 and -10-associated RING finger protein 1; E3 ubiquitin-protein ligase RNF34; EC 6.3.2; EC 6.3.2.-; FLJ21786; FYVE-RING finger protein Momo; hRFI; Human RING finger homologous to inhibitor of apoptosis protein; RFI; RIF; RIFF; ring finger protein 34CARP-1; RING finger protein RIFF RNF34 CARP-1, CARP1, RFI, RIF, RIFF, hRFI ring finger protein 34 E3 ubiquitin-protein ligase RNF34|FYVE-RING finger protein MOMO|RING finger protein RIFF|RING-type E3 ubiquitin transferase RNF34|caspase regulator CARP1|caspases-8 and -10-associated RING finger protein 1|human RING finger homologous to inhibitor of apoptosis protein|ring finger protein 34, E3 ubiquitin protein ligase

For more info on RNF34, check out the RNF34 Infographic

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In this infographic, you will see the following information for RNF34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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