Anti-RNF40 Antibody

Product Name

Anti-RNF40 Antibody

View all RNF40 Antibodies

SKU/Catalog Number

A06979-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RNF40 Antibody catalog # A06979-1. Tested in WB,IP,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06979-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human ADCY2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06979-1 is reactive to RNF40 in Human, Mouse, Rat

Applications

A06979-1 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

113.678kDa

Background of RNF40

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.

Celeste A.J., Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 434:724-731(2005).
Mazerbourg S., J. Biol. Chem. 280:32122-32132(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000
IP: 1:10-1:50
IHC: 1:50

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of RNF40 using Anti-RNF40 Antibody (A06979-1).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RNF40 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature

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Figure 1. Western blotting validation for Anti-RNF40 Antibody A06979-1

Western blot analysis of RNF40 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5

Gene/Protein Information For RNF40 (Source: Uniprot.org, NCBI)

Alternative Names

95 kDa retinoblastoma-associated protein; BRE1-B; BRE1BMGC13051; EC 6.3.2,95 kDa retinoblastoma protein binding protein; EC 6.3.2.-; KIAA0661BRE1 E3 ubiquitin ligase homolog B; Rb-associated protein; RBP95E3 ubiquitin-protein ligase BRE1B; ring finger protein 40DKFZp686K191; STARING RNF40 BRE1B, RBP95, STARING ring finger protein 40 E3 ubiquitin-protein ligase BRE1B|95 kDa retinoblastoma protein binding protein|95 kDa retinoblastoma-associated protein|BRE1 E3 ubiquitin ligase homolog B|BRE1-B|RING-type E3 ubiquitin transferase BRE1B|Rb-associated protein|ring finger protein 40, E3 ubiquitin protein ligase

For more info on RNF40, check out the RNF40 Infographic

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In this infographic, you will see the following information for RNF40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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