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- Table of Contents
RNF40 Antibodies
AND RNF40 ELISA kits, RNF40 Proteins
Many biological assays use antibodies to detect RNF40, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNF40 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNF40 antibodies...
Designing an IHC experiment for RNF40? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the RNF40 IHC GuideRNF40 Infographic by Boster Bio
All RNF40 Products
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RNF40 Overview
Facts about E3 ubiquitin-protein ligase BRE1B.
E3 ubiquitin-protein ligase BRE1B (RNF40)
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively).
It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports regarding the cooperation with UBE2E1/UBCH are contradictory.
Gene Information
| Human | |
|---|---|
| Gene Name: | RNF40 |
| Uniprot: | O75150 |
| Entrez: | 9810 |
Family
| Belongs to: |
|---|
| BRE1 family |
Alternative Names
95 kDa retinoblastoma-associated protein; BRE1-B; BRE1BMGC13051; EC 6.3.2,95 kDa retinoblastoma protein binding protein; EC 6.3.2.-; KIAA0661BRE1 E3 ubiquitin ligase homolog B; Rb-associated protein; RBP95E3 ubiquitin-protein ligase BRE1B; ring finger protein 40DKFZp686K191; STARING
Molecular Weight
Mass (kDA):
113.678 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 16p11.2 |
| Sequence: | 16; NC_000016.10 (30761612..30800231) |
Tissue Specificity
Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
Subcellular Localizations
Nucleus.
Diseases
- Neoplasms
- Malignant Neoplasms
- Carcinoma
- Genomic Instability
- Retinoblastoma
- Exposure To Ionizing Radiation
- Tumor Progression
- Malignant Neoplasm Of Breast
- Seminoma
- Glioma
- Gastrointestinal Neoplasms
- Head And Neck Carcinoma
Interacting Proteins
- HTT
- NCBP1
- RB1
- RNF20
- SFR1
Pathways
- Methylation
- Cell Cycle
- Cell Growth
- Cell Cycle Checkpoint
- Cell Proliferation
- Recombinational Repair
- Gene Silencing
- Stem Cell Differentiation
- Cell Cycle Phase
- Mrna 3'-end Processing
- Histone Monoubiquitination
- Dna Repair
- Histone Mrna 3'-end Processing
PTMs
- Methylation
- Phosphorylation
- Ubiquitination
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF40
bosterbio.com/bosterbio-gene-info-cards/RNF40
References
PMID: 10944455 by Wen H., et al. RBP95, a novel leucine zipper protein, binds to the retinoblastoma protein.
PMID: 16307923 by Zhu B., et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation.