Anti-RNF43 Antibody Picoband™

Product Name

Anti-RNF43 Antibody Picoband™

View all RNF43 Antibodies

SKU/Catalog Number

A01694-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF43 Antibody Picoband™ catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF43 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01694-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01694-1 is reactive to RNF43 in Human

Applications

A01694-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

86 kDa

Calculated molecular weight

85.722kDa

Background of RNF43

RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
Direct ELISA,0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.

Gene/Protein Information For RNF43 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase RNF43 RNF43 RNF124, SSPCS, URCC ring finger protein 43 E3 ubiquitin-protein ligase RNF43|RING-type E3 ubiquitin transferase RNF43

For more info on RNF43, check out the RNF43 Infographic

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In this infographic, you will see the following information for RNF43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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