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Product Info Summary
| SKU: | M05620 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-RNF7 Antibody (N-term)
SKU/Catalog Number
M05620
Size
50 µl
Description
Boster Bio Anti-RNF7 Antibody (N-term) (Catalog # M05620). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RNF7 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05620)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7.
Reactive Species
M05620 is reactive to RNF7 in Human, Mouse
Calculated molecular weight
12.7 kDa
Background of RNF7
Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05620 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RNF7 Antibody (N-term) at 1:8000 dilution
Lane 1: human heart lysates
Lane 2: human liver lysates
Lane 3: human skeletal muscle lysates
Lane 4: human testis lysates
Lane 5: mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 13 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M05620 staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-RNF7 Antibody (N-term) (M05620)
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