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Product Info Summary
| SKU: | A05085-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-RP105 CD180 Antibody
SKU/Catalog Number
A05085-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RP105 CD180 Antibody (Catalog # A05085-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
RP105 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RP105 CD180 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05085-1)
Host
Rabbit
Contents
RP105 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RP105 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RP105. The immunogen is located within amino acids 590 - 640 of RP105.
Reactive Species
A05085-1 is reactive to CD180 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
74.2 kDa
Background of CD180
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. It has also been suggested that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05085-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RP105 antibody can be used for the detection of RP105 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, RP105 often migrates at 95 - 105 kDa. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of RP105 in human spleen tissue withRP105 antibody at 10 μg/mL.
Specific Publications For Anti-RP105 CD180 Antibody (A05085-1)
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